Charge is the message: Non-covalent attachment to anionic supports (red) through a positively charged Zbasic2 (blue) module is a powerful approach for reversible oriented immobilization of enzymes (pI 4-7) on ion-exchange resins commonly used in the protein and enzyme technology. High protein loading and near-native catalytic effectiveness of the carrier-bound enzyme can be achieved.
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Wiesbauer et al. (2011) studied this question.
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