Immobilization of Strictosidine Synthase from Catharanthus Cell Cultures and Preparative Synthesis of Strictosidine

Strictosidine synthase was partially purified from Catharanthus roseus cell suspension cultures and immobilized on CNBr-activated Sepharose. The immobilized enzyme exhibits a thermostability increased 300 fold over that of the soluble enzyme and catalyses exclusively the formation of the 3α(S)-isomer, strictosidine. Gram quantities of this biologically active glucoalkaloid, which hitherto had been difficult to synthesize and purify, were prepared. Key Word Index Catharanthus roseus - Apocynaceae - Cell Culture - Strictosidine Synthase - Immobilized Enzyme - Strictosidine Preparation