Inclusion Complexes of Unsaturated Fatty Acids with Amylose and Cyclodextrin

The best method for determination of the composition of amylose‐fatty acid complexes comprises acid hydrolysis, followed by solvent exrtaction of the fatty acids. The fatty acid content of a cyclodextrin complex can be directly titrated with alcoholic alkali. The complexes were prepared in aqueous solution, and some influences of reaction parameters were observed. A higher fatty acid concentration resulted in every case in a higher fatty acid content of the complex. By washing the complex with dioxane or petroleum ether only the fatty acid adsorbed on its surface was removed. By using ethanol the fatty acid could be removed from the complex. The fraction of the fatty acid mixture incorporated into amylose is enriched in saturated fatty acids. The unsaturated fatty acids included into the complexes are practically completely protected against oxidation.