What question did this study set out to answer?

Determine whether targeting the bacterial polyhydroxybutyrate (PHB) biosynthetic pathway to plant plastids enables high-level polymer accumulation without hindering plant growth.

December 20, 1994Proceedings of the National Academy of SciencesOpen Access

Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.

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Authors

CNChristiane NawrathUniversity of Lausanne YPYves PoirierUniversity of Lausanne CSChristopher R. SomervillePortola Pharmaceuticals (United States)

Discussion

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Implication

Experimental study demonstrates high polyhydroxybutyrate accumulation in transgenic Arabidopsis plastids, indicating no biological barrier to plant-based bioplastic synthesis.

Key Points

Determine whether targeting the bacterial polyhydroxybutyrate (PHB) biosynthetic pathway to plant plastids enables high-level polymer accumulation without hindering plant growth.
Engineered three Alcaligenes eutrophus PHB biosynthetic genes with pea chloroplast transit peptides, constitutive plant promoters, and poly(A) addition sequences.
Transformed Arabidopsis thaliana via Agrobacterium-mediated transformation and performed sexual crosses to yield plants expressing all three enzymes.
Transgenic Arabidopsis accumulated PHB up to 14% of dry weight, sequestered as 0.2- to 0.7-micron granules inside plastids.
Targeting the pathway to plastids generated a 100-fold increase in PHB accumulation relative to cytosolic expression with no deleterious effects on plant growth or fertility.

Cite This Study

Nawrath et al. (1994) studied this question.

synapsesocial.com/papers/6a85bb6602a1bc3ea31e73ee https://doi.org/10.1073/pnas.91.26.12760

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December 20, 1994Proceedings of the National Academy of SciencesOpen Access

Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.

View Full Paper

Ask AI

Bookmark

Authors

CNChristiane NawrathUniversity of Lausanne YPYves PoirierUniversity of Lausanne CSChristopher R. SomervillePortola Pharmaceuticals (United States)

Discussion

Member takes

Implication

Experimental study demonstrates high polyhydroxybutyrate accumulation in transgenic Arabidopsis plastids, indicating no biological barrier to plant-based bioplastic synthesis.

Key Points

Determine whether targeting the bacterial polyhydroxybutyrate (PHB) biosynthetic pathway to plant plastids enables high-level polymer accumulation without hindering plant growth.
Engineered three Alcaligenes eutrophus PHB biosynthetic genes with pea chloroplast transit peptides, constitutive plant promoters, and poly(A) addition sequences.
Transformed Arabidopsis thaliana via Agrobacterium-mediated transformation and performed sexual crosses to yield plants expressing all three enzymes.
Transgenic Arabidopsis accumulated PHB up to 14% of dry weight, sequestered as 0.2- to 0.7-micron granules inside plastids.
Targeting the pathway to plastids generated a 100-fold increase in PHB accumulation relative to cytosolic expression with no deleterious effects on plant growth or fertility.

Cite This Study

Nawrath et al. (1994) studied this question.

synapsesocial.com/papers/6a85bb6602a1bc3ea31e73ee https://doi.org/10.1073/pnas.91.26.12760

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