What question did this study set out to answer?

To determine whether the cell-binding domains of high molecular weight kininogen, specifically domain 5, inhibit endothelial cell proliferation, migration, and angiogenesis.

January 15, 2000Blood

Domain 5 of high molecular weight kininogen (kininostatin) down-regulates endothelial cell proliferation and migration and inhibits angiogenesis

View Full Paper

Ask AI

Bookmark

Authors

RCRobert W. ColmanFilm Independent BJBradford A. JamesonAllegheny College YLYingzhang LinUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

Experimental study reveals that domain 5 of kininogen inhibits endothelial proliferation, migration, and angiogenesis, indicating a potent antiangiogenic role for kininostatin.

Key Points

To determine whether the cell-binding domains of high molecular weight kininogen, specifically domain 5, inhibit endothelial cell proliferation, migration, and angiogenesis.
Assayed endothelial cell migration toward vitronectin and cell proliferation in the presence of recombinant domains (D3, D5, D6) and derived synthetic peptides.
Mapped active inhibitory peptide sequences using deletion mutants, molecular homology modeling, and surface-loop peptide analogs.
Evaluated in vivo antiangiogenic activity using a chicken chorioallantoic membrane assay stimulated with fibroblast growth factor 2.
Domain 5 (D5) inhibited endothelial cell migration with an IC50 of 0.12 µM (85% inhibition at 0.27 µM) and proliferation with an IC50 of 30 nM, mapping minimal full activity to residues H441-D474.
Peptide 440-455 selectively suppressed endothelial cell proliferation with an IC50 of 100 nM without altering migration.
D5 inhibited fibroblast growth factor 2-stimulated angiogenesis by 97% at 270 nM in the chicken chorioallantoic membrane assay, while peptide 400-455 produced 79% inhibition.

Cite This Study

Colman et al. (2000) studied this question.

synapsesocial.com/papers/6a85bbb6159a1fb27a20317d https://doi.org/10.1182/blood.v95.2.543

View Full Paper

Ask AI

Bookmark

Implication

Experimental study reveals that domain 5 of kininogen inhibits endothelial proliferation, migration, and angiogenesis, indicating a potent antiangiogenic role for kininostatin.

Domain 5 of high molecular weight kininogen (kininostatin) down-regulates endothelial cell proliferation and migration and inhibits angiogenesis

Discussion

Member takes

Implication

Key Points

Cite This Study

Domain 5 of high molecular weight kininogen (kininostatin) down-regulates endothelial cell proliferation and migration and inhibits angiogenesis

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider