What question did this study set out to answer?

To determine the molecular mechanisms by which the flavonoids genistein, apigenin, and 3-hydroxyflavone inhibit growth-factor-induced angiogenesis in human endothelial cells.

April 24, 2003Journal of Cellular Biochemistry

Flavonoids inhibit VEGF/bFGF‐induced angiogenesis in vitro by inhibiting the matrix‐degrading proteases

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MKMyoung Hee KimUlsan College

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Implication

In vitro study demonstrates that flavonoids suppress growth-factor-induced angiogenesis in human endothelial cells, indicating a mechanism via matrix protease inhibition.

Key Points

To determine the molecular mechanisms by which the flavonoids genistein, apigenin, and 3-hydroxyflavone inhibit growth-factor-induced angiogenesis in human endothelial cells.
Treated serum-starved human umbilical vein endothelial cells (HUVECs) with vascular endothelial growth factor and basic fibroblast growth factor (VEGF/bFGF).
Pretreated cells with genistein, apigenin, or 3-hydroxyflavone to measure changes in matrix metalloproteinases (MMP-1, MT1-MMP, pro-MMP-2), uPA isoforms, and endogenous inhibitors (TIMP-1, TIMP-2, PAI-1).
Flavonoid pretreatment completely abolished the VEGF/bFGF-induced upregulation of MMP-1 and MT1-MMP expression as well as the activation of pro-MMP-2.
Flavonoids blocked the generation of the 33 kDa uPA fragment and decreased 55 kDa uPA activity and PAI-1 expression below basal levels while differentially regulating TIMP-1 and TIMP-2.

Cite This Study

Myoung Hee Kim (2003) studied this question.

synapsesocial.com/papers/6a85c0e4e954a56a77ada1bf https://doi.org/10.1002/jcb.10543

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Implication

In vitro study demonstrates that flavonoids suppress growth-factor-induced angiogenesis in human endothelial cells, indicating a mechanism via matrix protease inhibition.

Flavonoids inhibit VEGF/bFGF‐induced angiogenesis in vitro by inhibiting the matrix‐degrading proteases

Discussion

Member takes

Implication

Key Points

Cite This Study

Flavonoids inhibit VEGF/bFGF‐induced angiogenesis in vitro by inhibiting the matrix‐degrading proteases

Discussion

Member takes

Implication

Key Points

Cite This Study

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