Homogeneity of extracellular proteins of Trichoderma reesei found after isoelectric focusing (IEF) has been shown to reflect purity of a (multi)enzyme complex rather than purity of one enzyme isolated. The intention of the present study was to demonstrate to what extent distribution of (multi)enzyme complexes reflects a general concept of extracellular hydrolases in the culture fluid of T. reesei. The complete culture fluid studied by preparative IEF on the distribution of cellulases, β-glucosidase, xylanase and xylosidase confirms this view. (Macro)heterogeneity found after IEF is discussed on the basis of different charges of complexes.
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Sprey et al. (1984) studied this question.
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