Studies upon Calcification in vitro

THE object of these experiments was to determine the conditions under which calcification occurs in vitro, and also what factors interfere with calcification. If it can be shown that the position and the character of the calcification, in other words the histological picture, are exactly the same as those occurring in vivo then it would seem justifiable to draw certain deductions regarding in vivo calcification from in vitro experiments. Per contra, if certain factors prevent calcification in vitro, and if it can be shown that these same factors are operative in diseases such as rickets, in which there is a failure of calcifi- cation, then it would appear justifiable to explain the failure of calcification by the influence of these factors.