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Phenylalanyl ribonucleic acid synthetase has been isolated from Escherichia coli and is 93% pure as determined by analytical centrifugation and gel electrophoresis. The procedure is adaptable to large scale preparation. No other aminoacyl-RNA synthetases are present in the purified preparation. The molecular weight of the enzyme is 181,000, with an s20,w of 8.6. Optimal reaction conditions have been determined with highly purified phenylalanine transfer RNA that exhibits a Km of 2.6 x 10-7 m. The enzyme is stable when stored at 4°.
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M.P. Stulberg (1967) studied this question.
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