What question did this study set out to answer?

To determine whether human astrocytic genes involved in synaptic regulation display dynamic transcriptomic shifts associated with age, sex, and tumor disease states.

January 5, 2022Journal of NeuroscienceOpen Access

Human Astrocytes Exhibit Tumor Microenvironment-, Age-, and Sex-Related Transcriptomic Signatures

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Authors

MKMitchell C. Krawczyk JHJillian R. Haney LPLin Pan

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Overview

Transcriptomic analysis reveals downregulation of synaptic genes in peritumor and aging human cortical astrocytes, highlighting mechanisms of neural circuit dysfunction.

Key Points

To determine whether human astrocytic genes involved in synaptic regulation display dynamic transcriptomic shifts associated with age, sex, and tumor disease states.
Acutely isolated and purified cortical astrocytes from the cerebral cortices of over 40 human donors across diverse ages, sexes, and clinical states.
Performed RNA sequencing to profile the astrocyte transcriptome and detect differential gene expression correlated with biological variables.
Astrocytes within the peritumor microenvironment downregulated genes responsible for synaptic support and microenvironmental sensing.
Aging astrocytes exhibited reduced synaptic regulator expression accompanied by increased cytokine signaling markers, while developmental maturation altered ionic transport and calcium signaling pathways.
Transcriptomic profiling detected subtle sexual dimorphism in gene expression among human cortical astrocytes.

Cite This Study

Krawczyk et al. (2022) studied this question.

synapsesocial.com/papers/6a8645955dc285e07e739fc9 https://doi.org/10.1523/jneurosci.0407-21.2021

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January 5, 2022Journal of NeuroscienceOpen Access

Human Astrocytes Exhibit Tumor Microenvironment-, Age-, and Sex-Related Transcriptomic Signatures

View Full Paper

Ask AI

Bookmark

Authors

MKMitchell C. Krawczyk JHJillian R. Haney LPLin Pan

Discussion

Member takes

Overview

Transcriptomic analysis reveals downregulation of synaptic genes in peritumor and aging human cortical astrocytes, highlighting mechanisms of neural circuit dysfunction.

Key Points

To determine whether human astrocytic genes involved in synaptic regulation display dynamic transcriptomic shifts associated with age, sex, and tumor disease states.
Acutely isolated and purified cortical astrocytes from the cerebral cortices of over 40 human donors across diverse ages, sexes, and clinical states.
Performed RNA sequencing to profile the astrocyte transcriptome and detect differential gene expression correlated with biological variables.
Astrocytes within the peritumor microenvironment downregulated genes responsible for synaptic support and microenvironmental sensing.
Aging astrocytes exhibited reduced synaptic regulator expression accompanied by increased cytokine signaling markers, while developmental maturation altered ionic transport and calcium signaling pathways.
Transcriptomic profiling detected subtle sexual dimorphism in gene expression among human cortical astrocytes.

Cite This Study

Krawczyk et al. (2022) studied this question.

synapsesocial.com/papers/6a8645955dc285e07e739fc9 https://doi.org/10.1523/jneurosci.0407-21.2021

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