What question did this study set out to answer?

To determine the antiviral mechanism by which human MxA GTPase suppresses Thogoto virus replication across distinct cellular compartments using reconstituted viral ribonucleoprotein complexes.

January 1, 2000Journal of VirologyOpen Access

MxA GTPase Blocks Reporter Gene Expression of Reconstituted Thogoto Virus Ribonucleoprotein Complexes

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Authors

FWFriedemann WeberJustus-Liebig-Universität Gießen OHOtto HallerUniversity of Freiburg GKGeorg KochsUniversity Medical Center Freiburg

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Implication

In vitro study demonstrates MxA GTPase inhibition of Thogoto virus ribonucleoproteins in transfected cells, indicating distinct compartment-specific antiviral mechanisms.

Key Points

To determine the antiviral mechanism by which human MxA GTPase suppresses Thogoto virus replication across distinct cellular compartments using reconstituted viral ribonucleoprotein complexes.
Reconstituted recombinant Thogoto virus ribonucleoprotein complexes (vRNPs) from cloned cDNAs using two minireplicon reporter systems.
Expressed a chloramphenicol acetyltransferase minigenome using cytoplasmic T7 RNA polymerase or nuclear RNA polymerase I in transfected cells.
Compared the inhibitory effects of wild-type cytoplasmic MxA and engineered nuclear TMxA on viral reporter gene expression.
Both cytoplasmic MxA and nuclear TMxA selectively recognize assembled viral ribonucleoproteins rather than newly synthesized unassembled viral components.
Cytoplasmic MxA suppresses reporter gene expression by blocking the nuclear import of assembled vRNPs.
Nuclear TMxA directly inhibits viral polymerase activity within the host cell nucleus.

Cite This Study

Weber et al. (2000) studied this question.

synapsesocial.com/papers/6a8647772ffec2cf765ae6ab https://doi.org/10.1128/jvi.74.1.560-563.2000

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Implication

In vitro study demonstrates MxA GTPase inhibition of Thogoto virus ribonucleoproteins in transfected cells, indicating distinct compartment-specific antiviral mechanisms.

MxA GTPase Blocks Reporter Gene Expression of Reconstituted Thogoto Virus Ribonucleoprotein Complexes

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Key Points

Cite This Study

MxA GTPase Blocks Reporter Gene Expression of Reconstituted Thogoto Virus Ribonucleoprotein Complexes

Discussion

Member takes

Implication

Key Points

Cite This Study

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