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A quasioptical approach is utilized to design a resonator and sample holder for aqueous samples for high-frequency (250 GHz) electron spin resonance (ESR) spectroscopy. A disk shaped sample geometry was chosen to match the field contours of the fundamental mode in a Fabry–Perot resonator. A transmission-line analysis is used to determine the optimum sample geometry while taking into account diffractive effects of the sample and the sample holder on the far infrared (FIR) radiation field. FIR-ESR spectra of several aqueous solutions of nitroxide spin labels, including membrane vesicles and a labeled protein, are shown to demonstrate the success of the technique.
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Barnes et al. (1997) studied this question.
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