Paper Chromatographic Method for Estimation of Phenylalanine.

For paper chromatographic estimation of phenylalanine, 0.5 ml samples of blood serum are deproteinized with 4 volumes of 95% ethyl alcohol and the filtrate used for preparation of the chromatogram. The method is especially useful in study of phenylketonuric infants, and in following the changes in serum phenylalanine when a low-phenylalanine diet is used for their treatment. The recovery range of phenylalanine added to serum was from 80% to 106% with an average recovery of 95%.