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Hb D Los Angeles (B121 Glu-Gln) and Hb Beograd (B121 Glu-Val) can be identified by different techniques. Here we describe a dual restriction enzyme digestion protocol (EcoR IG AATTC) and Tsp509 I ( AATT) for the discrimination of these variants.
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Akar et al. (1995) studied this question.
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