What question did this study set out to answer?

To develop a method for characterizing the physicochemical properties of bile acid-lipid mixed micelles in the aqueous phase of human duodenal fluid during fat digestion.

October 1, 1975Journal of Clinical InvestigationOpen Access

Isolation and properties of the mixed lipid micelles present in intestinal content during fat digestion in man.

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Authors

CMC. M. MansbachUniversity of Tennessee Health Science Center RCR.S. CohenUniversity of Maryland, Baltimore PLP. B. LeffUnited States Department of Veterans Affairs

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Implication

Metabolic analysis demonstrates the isolation and composition of mixed lipid micelles in human duodenal fluid, indicating a precise stoichiometry during intestinal fat digestion.

Key Points

To develop a method for characterizing the physicochemical properties of bile acid-lipid mixed micelles in the aqueous phase of human duodenal fluid during fat digestion.
Duodenal fluid was obtained from humans after a 36-g fat breakfast across two 90-minute periods and 60 minutes following intravenous cholecystokinin.
The aqueous phase was separated using ultracentrifugation at 15,400,000 g-min, followed by filtration through 200-nm and 1.5-nm ultrafilters.
Micellar components were analyzed by Sepharose 6B gel permeation chromatography and sucrose density gradients.
Gel permeation chromatography displayed coincident peaks of bile acid, fatty acid, lysolecithin, and cholesterol, eluting with a Stokes radius of 2.3–3.5 nm (Kav 0.50–0.68).
Sucrose density gradient analysis revealed homogeneous mixed micelles with an average density of 1.25.
Stoichiometric regression analysis established that each mole of bile acid associated with 1.4 mol fatty acid, 0.15 mol lysolecithin, and 0.06 mol cholesterol.

Cite This Study

Mansbach et al. (1975) studied this question.

synapsesocial.com/papers/6a865d2091e65e8a392ecd79 https://doi.org/10.1172/jci108156

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Implication

Metabolic analysis demonstrates the isolation and composition of mixed lipid micelles in human duodenal fluid, indicating a precise stoichiometry during intestinal fat digestion.

Isolation and properties of the mixed lipid micelles present in intestinal content during fat digestion in man.

Discussion

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Implication

Key Points

Cite This Study

Isolation and properties of the mixed lipid micelles present in intestinal content during fat digestion in man.

Discussion

Member takes

Implication

Key Points

Cite This Study

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