Functional screening of somatic mutant events in extranodal natural killer/T-cell lymphoma with adrenal involvement

Figures

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  Figure 1. Immunohistochemical images of IgG, lgG4, lgGκ, and lgGλ in patients with left nasal sinusitis (scale bar: 25 μm).

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  Figure 2. Enhanced computed tomography images of the left adrenal mass in sagittal and coronal views (A, B) and three-dimensional construction images (C, D). 18F-FDG PET/CT of the patient (E).

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  Figure 3. Immunohistochemical images of CD3 (+), CD43 (+), CD56 (partial +), TIA (+), granzyme B (+), CD2 (+), CD4 (partial +), CD5 (–), CD7 (+), and CD8 (–) from a left adrenal sample obtained by laparoscopic resection.

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  Figure 4. Immunohistochemical images for CD20 (–), CD79α (–), CK (AE1/AE3) (–), and the 90% labeling index of Ki-67 in a left adrenal sample obtained via laparoscopic resection.

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  Figure 5. Immunohistochemical microscopic images of CD31 (partial +), CD34 (+), CD68 (+), CD163 (+), vimentin (+), SMA (+), PD-1 (–), PD-L1 (+) for the tumor micro-environment analysis in the tumor sample.

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  Figure 6. Overall survival curves of patients with extranodal natural killer/T-cell lymphoma (ENKTL) and adrenocortical carcinoma (ACC).

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  Figure 7. Forest plot of the univariate regression analysis (A) and multivariate regression analysis (B) revealing the relationships between different prognostic factors and overall survival in extranodal natural killer/T-cell lymphoma (ENKTL) patients (n=39). The tetragonal diamonds represent the hazard ratios (HRs), and the horizontal line crossing the diamonds represents the 95% confidence intervals (CIs). The data were analyzed by a Cox proportional hazards model and are shown as HRs and 95% CIs.

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  Figure 8. Pie chart representing the distribution of the number of INDELs. (A) The left and right images show the number of INDELs in different regions of the genome and in coding regions in tumor tissue, respectively. (B) The left and right images show the number of INDELs in different regions of the genome and the coding regions in normal tissue, respectively.

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  Figure 9. Genomic variation Circos plot. The structure of the three layers that run from the outside to the inside represents the sequence coverage map, SNP and INDEL density, and CNV results, respectively. Red indicates an increase in the copy number, blue indicates a decrease in the copy number, and green indicates a normal copy number.

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  Figure 10. Sanger sequencing electropherograms of the STAT3 mutant at position chr17: 40474482 A>T (A), TET2 mutant at position chr4: 106157174 G>A (B), FAS mutant at position chr10: 90773993 G>A (C) and TP53 mutant at position chr17: 7578394 C>T (D).

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  Figure 11. Alteration frequency of STAT3, TET2, FAS and TP53 in different studies.

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  Figure 12. Lollipop plots of gene mutations. (A) STAT3. (B) TET2. (C) FAS. (D) TP53.