What question did this study set out to answer?

To determine whether and how the orphan nuclear receptor Nurr1 directly regulates the transcription and promoter activity of the tyrosine hydroxylase (TH) gene.

March 28, 2003Journal of NeurochemistryOpen Access

Orphan nuclear receptor Nurr1 directly transactivates the promoter activity of the tyrosine hydroxylase gene in a cell‐specific manner

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Authors

KKKwang‐Soo KimWashington University in St. Louis CKChun‐Hyung KimAssociation of Southeast Asian Nations DHDong‐Youn HwangCHA University

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Implication

In vitro molecular assays demonstrate Nurr1 activation of the tyrosine hydroxylase promoter in dopaminergic cells, indicating a direct mechanism for dopamine neuron specification.

Key Points

To determine whether and how the orphan nuclear receptor Nurr1 directly regulates the transcription and promoter activity of the tyrosine hydroxylase (TH) gene.
Assessed promoter transactivation of tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH) across different cell types using transient transfection reporter assays.
Identified functional DNA regulatory elements through promoter deletion, site-directed mutagenesis, DNase I footprinting, and electrophoretic mobility shift assays (EMSA).
Nurr1 directly transactivated the TH promoter in a cell type-dependent manner without affecting the DBH promoter, mediated by upstream sequences under 1.0 kb containing three NBRE-like motifs.
Mutational analysis showed that motif NL1 (-35 to -28 bp) was essential for transactivation, whereas binding assays revealed that NL3 exhibited the highest direct binding affinity to Nurr1 and maximal activity when positioned proximal to the TATA box.

Cite This Study

Kim et al. (2003) studied this question.

synapsesocial.com/papers/6a86639dfb63d0a2a03a7291 https://doi.org/10.1046/j.1471-4159.2003.01671.x

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Implication

In vitro molecular assays demonstrate Nurr1 activation of the tyrosine hydroxylase promoter in dopaminergic cells, indicating a direct mechanism for dopamine neuron specification.

Orphan nuclear receptor Nurr1 directly transactivates the promoter activity of the tyrosine hydroxylase gene in a cell‐specific manner

Discussion

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Implication

Key Points

Cite This Study

Orphan nuclear receptor Nurr1 directly transactivates the promoter activity of the tyrosine hydroxylase gene in a cell‐specific manner

Discussion

Member takes

Implication

Key Points

Cite This Study

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