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July 27, 2023Journal of Interferon & Cytokine Research

Characterization of Monoclonal Antibodies to Measure Cell Surface Protein Levels of Human Interferon-Lambda Receptor 1

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Why the study?

Conflicting data exist regarding which cells express IFN-lambdaR1, partly because IFNLR1 transcript levels do not always correlate with cell surface protein levels.

Population

Cell lines and primary human blood cells, including plasmacytoid dendritic cells and B cells

Comparison

Novel monoclonal antibodies tested for binding and detection of human IFN-lambdaR1

Design

In vitro laboratory study

Authors

NWNicole A. de WeerdHudson Institute of Medical ResearchOOOlamide OgungbolaUniversity of ManitobaXLXinyun LiuXuzhou Medical College

Discussion

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Implication

May refine IFN-λ therapeutic targeting; leaves open precise leukocyte subsets mediating responses.

Structured PICO

P
Population
Cell lines and primary human blood cells (plasmacytoid dendritic cells and B cells from peripheral blood)
I
Intervention
Novel monoclonal antibodies (HLR1 and HLR14) targeting human IFN-λR1
O
Outcome
Detection of cell surface IFN-λR1 protein by flow cytometry and ELISAsurrogate

The HLR14 monoclonal antibody enables the quantification of IFN-λR1 protein levels on cells, facilitating better characterization of cell-specific IFN-λ responses.

Cite This Study

Weerd et al. (2023) studied this question.

synapsesocial.com/papers/6a8667ef9d0eab71624ca58fhttps://doi.org/10.1089/jir.2023.0040
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Type III interferons: Balancing tissue tolerance and resistance to pathogen invasion2019 · 162 citations
  2. 2Differential expression of interferon-lambda receptor 1 splice variants determines the magnitude of the antiviral response induced by interferon-lambda 3 in human immune cells2020 · 63 citations
  3. 3Identification of the Functional Interleukin-22 (IL-22) Receptor Complex2001 · 401 citations
  4. 4Immune Cell Profiling of IFN-λ Response Shows pDCs Express Highest Level of IFN-λR1 and Are Directly Responsive via the JAK-STAT Pathway2016 · 51 citations
  5. 5Influence of Canonical and Non-Canonical IFNLR1 Isoform Expression on Interferon Lambda Signaling2023 · 12 citations