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Yanagimachi (1972a) published the first report of capacitation in vitro of guinea-pig spermatozoa. He incubated the spermatozoa in a medium developed by Biggers, Whitten & Whittingham (1971). After 12 to 18 hr incubation, the spermatozoa showed evidence of the acrosomal reaction and were able to penetrate guinea-pig eggs in vitro, with subsequent dispersion of the sperm head and pronuclear formation. So far, all the systems assayed for capacitation in vitro of mammalian spermatozoa have in their composition complex macromolecules such as those present in the body fluids or blood serum fractions. We decided to undertake a further investigation of the conditions under which guinea-pig spermatozoa would undergo capacitation. For this purpose, the epididymal tails were removed from adult guinea-pigs and the epididymal spermatozoa were suspended in 0·9% sodium chloride. From this sperm suspension,
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Barros et al. (1973) studied this question.
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