Mapping the lymphatic system across body scales and expertise domains: A report from the 2021 National Heart, Lung, and Blood Institute workshop at the Boston Lymphatic Symposium

Figures

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  FIGURE 1. The lower extremity of a cadaver injected with dye into the superficial lymphatic vessels shows the medial pathway (left) and posterior pathway running along the small saphenous vein (right).

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  FIGURE 2. Lymphosomes of the body. The lymphatic territories are demarcated according to their corresponding lymphatic basins: 1. Temporal, 2. Occipital, 3. Superficial cervical, 4. Subclavicular, 5. Subscapular, 6. Lateral axillary, 7. Pectoral, 8. Superior inguinal, 9. Lateral inguinal, 10. Inferior inguinal, 11. Popliteal.

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  FIGURE 3. The fluoroscopic image of the intranodal lymphangiography that is performed by placing a needle (arrow) inside the inguinal lymph node (black arrowhead) and injecting oil based iodinated contrast. The efferent lymphatics and distal lymph nodes (white arrowheads) are opacified.

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  FIGURE 4. Fifty-plex confocal images of a human mesenteric lymph node obtained by the IBEX method. Two to four marker overlays for two regions (germinal center, white rectangle and medullary cords, red rectangle) are shown in higher zoom. Scale bars, 500 and 100 μm, for the overview and zoom-in images, respectively. β-Tubulin 3 (β-Tub3), collagen IV (Coll IV), fibronectin (Fibro), laminin (Lamin), and vimentin (Viment). Figure kindly provided by Nature Methods (Hickey et al., 2022).