Adaptive strategies of the influenza virus polymerase for replication in humans
Population
Human and porcine cells, avian and swine-origin influenza viruses
Comparison
Introduction of second-site suppressor mutations… vs Wild-type avian polymerase
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Alerts clinicians to monitor PB2 mutations in emerging influenza strains; extends molecular understanding of host adaptation but leaves open real-world implications.
Structured PICO
P
Population
Human and porcine cells, avian and swine-origin influenza viruses (including 2009 H1N1 and primary avian isolates)
I
Intervention
Introduction of second-site suppressor mutations in the PB2 polymerase subunit or introduction of a human PA subunit
C
Comparator
Wild-type avian polymerase (with glutamic acid at amino acid 627)
O
Outcome
Polymerase activity and viral replication in human and porcine cellssurrogate
The study identifies adaptive strategies, such as second-site suppressor mutations in the PB2 subunit or introduction of a human PA subunit, that allow avian and swine-origin influenza viruses to overcome species-specific restriction and enhance replication in human cells.
Cite This Study
Mehle et al. (2009) studied this question.