What question did this study set out to answer?

To characterize changes in membrane water permeability, heats of activation, and osmotically active water volume in Ehrlich ascites tumor cells throughout the cell cycle.

December 1, 1978Journal of Cellular Physiology

Osmotic properties of ehrlich ascites tumor cells during the cell cycle

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Authors

ADAnn DuPreMedical University of South Carolina HHH. G. HemplingMedical University of South Carolina

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Implication

In vitro study demonstrates dynamic shifts in water permeability and osmotically active water across cell cycle phases, suggesting changes in membrane fluidity and bound water.

Key Points

To characterize changes in membrane water permeability, heats of activation, and osmotically active water volume in Ehrlich ascites tumor cells throughout the cell cycle.
Cultured Ehrlich ascites tumor cells in continuous spinner culture and synchronized them using a double thymidine block technique.
Identified cell cycle phases by tracking mitotic index, cell count, and DNA synthesis over time.
Measured membrane permeability to water, activation energy (heats of activation), total water content, major ion concentrations (Na, K, Cl), and Ponder's R.
Water permeability peaked at the start of the S phase and declined to its lowest level post-mitosis, with activation energies ranging between 9 and 14 kcal/mole.
Total cell water content stayed stable at 82% (w/w) and major ion concentrations remained constant, but the osmotically active water fraction (Ponder's R) dropped from 0.75 in S phase to approximately 0.56 after mitosis.
The volume of osmotically active cell water peaked during S and early G2 before dropping during mitosis, indicating that an increasing fraction of intracellular water becomes bound as cells divide.

Cite This Study

DuPre et al. (1978) studied this question.

synapsesocial.com/papers/6a8693938ce577d54a0cfb27 https://doi.org/10.1002/jcp.1040970313

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Implication

In vitro study demonstrates dynamic shifts in water permeability and osmotically active water across cell cycle phases, suggesting changes in membrane fluidity and bound water.

Osmotic properties of ehrlich ascites tumor cells during the cell cycle

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Implication

Key Points

Cite This Study

Osmotic properties of ehrlich ascites tumor cells during the cell cycle

Discussion

Member takes

Implication

Key Points

Cite This Study

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