Per Operative Trypan Blue Vital Staining of Corneal Endothelium

An 0.1% aqueous trypan blue solution was injected into the anterior chamber during cataract extraction for the purpose of disclosing endothelial cell damage if present. Follow-up examination of 24 patients after eight years revealed no side-effects of trypan blue, as judged by corneal opacities, compared with non-vital-stained aphakic and non-operated control eyes.