What question did this study set out to answer?

Investigate how Compound Sophorae Decoction preserves intestinal barrier function in dextran sodium sulfate-induced colitis and explore the role of the Notch signaling pathway.

November 18, 2020Biomedicine & PharmacotherapyOpen Access

Compound sophorae decoction enhances intestinal barrier function of dextran sodium sulfate induced colitis via regulating notch signaling pathway in mice

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Authors

HWHui WuQingdao University QCQian-Yun ChenUnion Hospital WWWenzhu WangChinese Academy of Sciences

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Implication

Animal study reveals Compound Sophorae Decoction restores intestinal barrier integrity in DSS-induced colitis mice, suggesting therapeutic efficacy via Notch pathway modulation.

Key Points

Investigate how Compound Sophorae Decoction preserves intestinal barrier function in dextran sodium sulfate-induced colitis and explore the role of the Notch signaling pathway.
Induced acute colitis in C57BL/6J mice using oral 3% dextran sodium sulfate (DSS) alongside concurrent oral administration of Compound Sophorae Decoction (CSD).
Recorded clinical parameters (body weight, stool), measured colon length, and assessed epithelial apoptosis, proliferation, and mucosal permeability using cleaved caspase-3, Ki-67, and FITC-dextran 4000.
Analyzed tight junction proteins (ZO-1, occludin), MUC2, and Notch pathway factors via western blot, and evaluated splenic and mesenteric lymph node macrophage M1/M2 polarization.
CSD significantly mitigated clinical disease activity, prevented body weight loss, inhibited colon shortening, and lowered intestinal permeability to FITC-dextran.
Treatment reduced colonic epithelial apoptosis while promoting cell proliferation, alongside marked upregulation of tight junction markers ZO-1 and occludin and increased MUC2 secretion.
CSD decreased the pro-inflammatory M1/M2 macrophage ratio and modulated the Notch signaling cascade in colonic tissue.

Cite This Study

Wu et al. (2020) studied this question.

synapsesocial.com/papers/6a86b541540b033b21beec19 https://doi.org/10.1016/j.biopha.2020.110937

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Implication

Animal study reveals Compound Sophorae Decoction restores intestinal barrier integrity in DSS-induced colitis mice, suggesting therapeutic efficacy via Notch pathway modulation.

Compound sophorae decoction enhances intestinal barrier function of dextran sodium sulfate induced colitis via regulating notch signaling pathway in mice

Discussion

Member takes

Implication

Key Points

Cite This Study

Compound sophorae decoction enhances intestinal barrier function of dextran sodium sulfate induced colitis via regulating notch signaling pathway in mice

Discussion

Member takes

Implication

Key Points

Cite This Study

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