What question did this study set out to answer?

To optimize the second-generation production of polyhydroxybutyrate (PHB) biopolymer from residual corn lignocellulosic fractions using a one-pot pretreatment and enzymatic hydrolysis strategy.

August 20, 2026Biotechnology and Applied BiochemistryOpen Access

Second‐Generation Polyhydroxybutyrate Production From Pretreated Corn Lignocellulosic Fraction as a Strategy to Repurpose by Products From the Corn‐Processing Chain

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Authors

CMClara Matte Borges Machado LVLuciana Porto de Souza Vandenberghe AMAriane Fátima Murawski de Mello

Discussion

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Overview

Experimental study demonstrates efficient bioplastic production from corn waste using an integrated pretreatment method, suggesting a cost-effective route for sustainable biorefineries.

Key Points

To optimize the second-generation production of polyhydroxybutyrate (PHB) biopolymer from residual corn lignocellulosic fractions using a one-pot pretreatment and enzymatic hydrolysis strategy.
Conducted a one-pot integrated citric acid pretreatment and enzymatic hydrolysis in an agitated tank using 11.64 mg β-glucosidase/g substrate and 0.6 mg cellulase/g substrate.
Cultivated Cupriavidus necator LPB2327 using the generated corn hydrolysate as a low-cost carbon source for PHB synthesis under optimized fermentation conditions.
Hydrolysis reached 47.37 g/L of total sugars, with glucose comprising 95.71% of the sugar content.
Fermentation produced 8.90 g/L of dry cell weight and 7.64 g/L of PHB, corresponding to an intracellular accumulation of 85.89%.
The overall conversion process achieved product yields of 0.254 g PHB/g substrate and 0.127 g PHB/g lignocellulosic corn fraction.

Cite This Study

Machado et al. (2026) studied this question.

synapsesocial.com/papers/6a86b5528a91293e6a1cc9f6 https://doi.org/10.1002/bab.70197

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August 20, 2026Biotechnology and Applied BiochemistryOpen Access

Second‐Generation Polyhydroxybutyrate Production From Pretreated Corn Lignocellulosic Fraction as a Strategy to Repurpose by Products From the Corn‐Processing Chain

View Full Paper

Ask AI

Bookmark

Authors

CMClara Matte Borges Machado LVLuciana Porto de Souza Vandenberghe AMAriane Fátima Murawski de Mello

Discussion

Member takes

Overview

Experimental study demonstrates efficient bioplastic production from corn waste using an integrated pretreatment method, suggesting a cost-effective route for sustainable biorefineries.

Key Points

To optimize the second-generation production of polyhydroxybutyrate (PHB) biopolymer from residual corn lignocellulosic fractions using a one-pot pretreatment and enzymatic hydrolysis strategy.
Conducted a one-pot integrated citric acid pretreatment and enzymatic hydrolysis in an agitated tank using 11.64 mg β-glucosidase/g substrate and 0.6 mg cellulase/g substrate.
Cultivated Cupriavidus necator LPB2327 using the generated corn hydrolysate as a low-cost carbon source for PHB synthesis under optimized fermentation conditions.
Hydrolysis reached 47.37 g/L of total sugars, with glucose comprising 95.71% of the sugar content.
Fermentation produced 8.90 g/L of dry cell weight and 7.64 g/L of PHB, corresponding to an intracellular accumulation of 85.89%.
The overall conversion process achieved product yields of 0.254 g PHB/g substrate and 0.127 g PHB/g lignocellulosic corn fraction.

Cite This Study

Machado et al. (2026) studied this question.

synapsesocial.com/papers/6a86b5528a91293e6a1cc9f6 https://doi.org/10.1002/bab.70197

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