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Establish an integrated optimization framework combining nutrient regulation, transformation timing, and transcriptomic profiling to overcome transformation recalcitrance in peanut.

August 20, 2026Industrial Crops and ProductsOpen Access

Integrated optimization of somatic embryogenesis and genetic transformation reveals novel regulators of regeneration competence in peanut

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Authors

SWShijie Wang AYAyixianmuguli Yishake YLYantong LaiSouth China Normal University

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Implication

Experimental study demonstrates optimized nutrient ratios and developmental timing overcome genetic transformation recalcitrance in peanut cultivars, highlighting key morphogenic regulators.

Key Points

Establish an integrated optimization framework combining nutrient regulation, transformation timing, and transcriptomic profiling to overcome transformation recalcitrance in peanut.
Screened somatic embryogenesis (SE) capacity across 12 peanut cultivars and optimized carbon-to-nitrogen (C/N) and nitrate-to-ammonium ratios.
Identified the optimal developmental window for Agrobacterium-mediated infection during SE induction.
Performed transcriptomic profiling during induction stages and functionally characterized the ATS3B gene family via overexpression and gene silencing.
Fine-tuning the C/N ratio (1.4 C:1 N, NO₃⁻/NH₄⁺ ≈4.4:1) raised SE rates in low-responsive cultivars from below 30% to over 85%, while cultivar NDH108 achieved induction rates exceeding 90%.
Identified an optimal Agrobacterium infection window approximately one month post-induction and observed metabolic reprogramming linked to light-to-dark culture transitions.
Demonstrated that ATS3B expression correlates with auxin transporters PIN1 and PIN3, where ATS3B overexpression promotes well-organized embryo development and silencing causes chaotic callus proliferation.

Cite This Study

Wang et al. (2026) studied this question.

synapsesocial.com/papers/6a86b5da8a91293e6a1cd611 https://doi.org/10.1016/j.indcrop.2026.124175

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Implication

Experimental study demonstrates optimized nutrient ratios and developmental timing overcome genetic transformation recalcitrance in peanut cultivars, highlighting key morphogenic regulators.

Integrated optimization of somatic embryogenesis and genetic transformation reveals novel regulators of regeneration competence in peanut

Discussion

Member takes

Implication

Key Points

Cite This Study

Integrated optimization of somatic embryogenesis and genetic transformation reveals novel regulators of regeneration competence in peanut

Discussion

Member takes

Implication

Key Points

Cite This Study

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