Recruitment and Immobilization of a Fluorinated Biomarker Across an Interfacial Phospholipid Film using a Fluorocarbon Gas

Perfluorohexane gas when introduced in the air atmosphere above a film of phospholipid self‐supported on an aqueous solution of C 2 F 5 ‐labeled compounds causes the recruitment and immobilization of the latter in the interfacial film. When the phospholipid forms a liquid‐condensed Gibbs monolayer, which is the case for dipalmitoylphosphatidylcholine (DPPC), the C 2 F 5 ‐labeled molecule remains trapped in the monolayer after removal of F ‐hexane. Investigations involve bubble profile analysis tensiometry (Gibbs films), Langmuir monolayers and microbubble experiments. The new phenomenon was utilized to incorporate a hypoxia biomarker, a C 2 F 5 ‐labeled nitrosoimidazole (EF5), in microbubble shells. This finding opens perspectives in the delivery of fluorinated therapeutic molecules and biomarkers.