Key result
Gegen Qilian Decoction abated NASH-associated liver injuries in HFD-fed rats and cell models via anti-oxidative stress and anti-inflammatory responses involving inhibition of TLR4 signaling.
Why the study?
GGQLD is a well-established Chinese medicine prescription for nonalcoholic steatohepatitis, but its underlying molecular mechanisms remain unclear.
Does Gegen Qinlian Decoction abate nonalcoholic steatohepatitis associated liver injuries in HFD-fed rats and cell models?
Does Gegen Qinlian Decoction abate nonalcoholic steatohepatitis associated liver injuries in HFD-fed rats and cell models?
Gegen Qinlian Decoction abates NASH-associated liver injuries through anti-oxidative and anti-inflammatory mechanisms involving the inhibition of TLR4 signaling pathways.
GGQLD lipid reductions in rat NASH models; hypothesis-generating, requiring human trials before any clinical consideration.
Gegen Qilian Decoction (GGQLD) is a well-established classic Chinese medicine prescription in treating nonalcoholic steatohepatitis (NASH). However, the molecular mechanism of GGQLD action on NASH is still not clear. This study aimed to assess the anti-NASH effect of GGQLD, and to explore its molecular mechanisms in vivo and in vitro. In HFD-fed rats, GGQLD decreased significantly serum triglyceride (TG), cholesterol (CHO), total bile acid (TBA), low-density lipoprotein (LDL), free fatty acid (FFA) and lipopolysaccharide (LPS) levels, increased levels of differentially expressed proteins (DEPs) Ahcy, Gpx1, Mat1a, GNMT, and reduced the expression of ALDOB. In RAW264.7 macrophages, GGQLD reduced the expression levels of inflammatory factors TNF-α and IL-6 mRNA, and diminished NASH by increasing differentially expressed genes (DEGs) CBS, Mat1a, Hnf4α and Pparα to reduce oxidative stress or lipid metabolism. The results of DEGs verification also showed that GGQLD up-regulated expressions of Hnf4α, Pparα and Cbs genes. In HepG2 cells, GGQLD decreased IL-6 levels and intracellular TG content, and inhibited FFA-induced expression of toll-like receptor 4 (TLR4). In summary, GGQLD abates NASH associated liver injuries via anti-oxidative stress and anti-inflammatory response involved inhibition of TLR4 signal pathways. These findings provide new insights into the anti-NASH therapy by GGQLD.
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Zhang et al. (2020) studied nonalcoholic steatohepatitis (NASH). Gegen Qilian Decoction (GGQLD) was evaluated on NASH associated liver injuries. Gegen Qilian Decoction abated NASH-associated liver injuries in HFD-fed rats and cell models via anti-oxidative stress and anti-inflammatory responses involving inhibition of TLR4 signaling.
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