What question did this study set out to answer?

To evaluate the potential for plasmid transduction in Pseudomonas aeruginosa within a natural freshwater habitat, assessing the impact of donor cell concentration and the native microbial community.

May 1, 1987Applied and Environmental MicrobiologyOpen Access

Potential for transduction of plasmids in a natural freshwater environment: effect of plasmid donor concentration and a natural microbial community on transduction in Pseudomonas aeruginosa

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Authors

DSD J SayeLoyola University Chicago OOOladele A. OgunseitanUniversity of California, Irvine GSGary S. SaylerUniversity of Tennessee at Knoxville

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Implication

Experimental study demonstrates bacteriophage-mediated plasmid transduction in Pseudomonas aeruginosa within freshwater, indicating donor concentration influences environmental gene transfer.

Key Points

To evaluate the potential for plasmid transduction in Pseudomonas aeruginosa within a natural freshwater habitat, assessing the impact of donor cell concentration and the native microbial community.
Inoculated environmental test chambers submerged in a freshwater reservoir with nonlysogenic plasmid donor cells and bacteriophage phi DS1 lysogens serving as phage sources and recipients.
Maintained recipient cells at natural environmental densities over a 9-day incubation period while altering donor cell concentrations both in the presence and absence of native microbes.
Generalized transducing bacteriophage phi DS1 successfully transferred plasmid Rms149 to recipient bacteria during the 9-day freshwater incubation.
Increasing the concentration of plasmid donor cells significantly increased the frequency of transduction events.
The presence of the natural microbial community rapidly decreased surviving donor and recipient cell counts, resulting in reduced numbers of recovered transductants.

Cite This Study

Saye et al. (1987) studied this question.

synapsesocial.com/papers/6a86c654c7caab105a1544dd https://doi.org/10.1128/aem.53.5.987-995.1987

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Implication

Experimental study demonstrates bacteriophage-mediated plasmid transduction in Pseudomonas aeruginosa within freshwater, indicating donor concentration influences environmental gene transfer.

Potential for transduction of plasmids in a natural freshwater environment: effect of plasmid donor concentration and a natural microbial community on transduction in Pseudomonas aeruginosa

Discussion

Member takes

Implication

Key Points

Cite This Study

Potential for transduction of plasmids in a natural freshwater environment: effect of plasmid donor concentration and a natural microbial community on transduction in Pseudomonas aeruginosa

Discussion

Member takes

Implication

Key Points

Cite This Study

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