Ethanol is typically used as a stabilizer in chloroform. Failure to remove this ethanol from the chloroform used in the extraction of lipids leads subsequently to the formation of ethyl ester artifacts during the preparation of methyl esters by a commonly employed transesterification procedure. Depending on the conditions and phases used during gas-liquid chromatography, the ethyl esters may be resolved from the corresponding methyl esters. The resulting chromatograms contain extraneous peaks and may be incorrectly identified.
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Johnson et al. (1976) studied this question.
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