What is the clinical evidence from this study?

Study design: Other. Population: Asthma (in vitro model). Intervention: Compressive stress vs. Unstimulated controls. Primary outcome: Percentage of cells staining positively for MUC5AC protein (p=<0.05).

January 23, 2009American Journal of Respiratory Cell and Molecular BiologyOpen Access

Chronic Intermittent Mechanical Stress Increases MUC5AC Protein Expression

Q: What are the key findings of this study?

Compressive stress applied for 1 hour per day for 14 days significantly increased the percentage of cells staining positively for MUC5AC protein to 22.0% compared to 8.6% in unstimulated controls.

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Key result

Compressive stress applied for 1 hour per day for 14 days significantly increased the percentage of cells staining positively for MUC5AC protein to 22.0% compared to 8.6% in unstimulated controls.

Why the study?

Does chronic intermittent compressive stress increase MUC5AC protein expression in normal human bronchial epithelial cells?

Population

Normal human bronchial epithelial cells cultured at an air-liquid interface for 14 days to establish a…

Comparison

Chronic, intermittent compressive stress applied… vs Time-matched unstimulated control cells…

Design

Preclinical, Microscopist blinded to sample conditions for image acquisition

Follow-up

14 days

Authors

JPJin‐Ah ParkHarvard University DTDaniel J. TschumperlinMayo Clinic

Discussion

Member takes

Implication

May link bronchoconstriction to mucus hypersecretion in asthma; leaves open in vivo relevance and therapeutic targeting.

Structured PICO

Does chronic intermittent compressive stress increase MUC5AC protein expression in normal human bronchial epithelial cells?

Population

Normal human bronchial epithelial cells cultured at an air-liquid interface to establish a mature mucociliary phenotype.

Intervention

Chronic, intermittent compressive stress (30 cm H2O) applied for 10 minutes or 1 hour per day for up to 14 days, with or without EGFR inhibitor (AG1478) and TGF-beta2 neutralizing antibody

Comparator

Time-matched unstimulated control cells maintained at atmospheric pressure

Outcome

Percentage of cells staining positively for MUC5AC protein and intracellular MUC5AC protein levelssurrogate

Main Result

Absolute Event Rate: 22% vs 8.6%

p-value: p=<0.05

Short duration episodes of mechanical stress, representative of bronchoconstriction in asthma, are sufficient to increase goblet cell number and MUC5AC protein expression in bronchial epithelial cells in vitro via EGFR and TGF-beta2 signaling.

Limitations

In vitro study design may not fully replicate in vivo human asthma conditions.
Lack of robust changes in transcript levels for MUC5AC and other epithelial phenotypic markers after 14 days of compressive stress.

Cite This Study

Park et al. (2009) studied Asthma (in vitro model). Compressive stress vs. Unstimulated controls was evaluated on Percentage of cells staining positively for MUC5AC protein (p=<0.05). Compressive stress applied for 1 hour per day for 14 days significantly increased the percentage of cells staining positively for MUC5AC protein to 22.0% compared to 8.6% in unstimulated controls.

synapsesocial.com/papers/6a86d5677fdf9298f89eb78d https://doi.org/10.1165/rcmb.2008-0195oc

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Chronic Intermittent Mechanical Stress Increases MUC5AC Protein Expression

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Chronic Intermittent Mechanical Stress Increases MUC5AC Protein Expression

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Also Consider

Also Consider