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April 28, 2025Animal BioscienceOpen Access

Discovery and functional study of lncRNAs associated with fat deposition in Kele pigs based on whole-transcriptome RNA sequencing

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Key result

RNA-seq revealed 493 differentially expressed lncRNAs, with TCONS_00161198 promoting fat deposition and TCONS_00185937 potentially facilitating fat degradation in pig subcutaneous fat precursor cells.

Why the study?

This study was conducted to identify lncRNAs associated with fat deposition in Kele pigs and investigate their molecular regulatory mechanisms.

Population

Six healthy 10-month-old male Kele pigs

Comparison

High backfat thickness (>5%, H) vs low backfat thickness (<5%, L) group

Design

Preclinical whole-transcriptome RNA-sequencing and in vitro functional study

Authors

XZXiong ZhangRYRunqian YangZAZhaobi Ai

Discussion

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Overview

Identifies candidate lncRNAs for porcine fat deposition; leaves open any role in human cardiometabolic disease.

Structured PICO

P
Population
6 healthy 10-month-old male Kele pigs divided into high and low backfat thickness groups to study lncRNAs associated with fat deposition.
E
Exposure
Transfection with pEGFP-TCONS_00185937 or pEGFP-TCONS_00161198
C
Comparator
Control group (pEGFP-N3 empty vector)
O
Outcome
Expression levels of target genes (MOGAT2, ATGL, CSF3R, ACC) and triglyceride content/fat depositionsurrogate

Main Result

p-value: p=<0.05

TCONS_00161198 and TCONS_00185937 regulate target genes to affect fat deposition in pig subcutaneous fat precursor cells, providing a theoretical basis for regulating fat deposition in Kele pigs.

Cite This Study

Zhang et al. (2025) studied Fat deposition (n=6). High backfat thickness vs. Low backfat thickness was evaluated on Differentially expressed lncRNAs (p=<0.05). RNA-seq revealed 493 differentially expressed lncRNAs, with TCONS_00161198 promoting fat deposition and TCONS_00185937 potentially facilitating fat degradation in pig subcutaneous fat precursor cells.

synapsesocial.com/papers/6a86d5fc713eb98b40ca0fe3https://doi.org/10.5713/ab.24.0900
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