What question did this study set out to answer?

To assess three methods of quantifying proliferating cell nuclear antigen (PCNA) immunostaining in astrocytic neoplasms and establish their diagnostic feasibility and correlation with patient survival.

November 1, 1993The Journal of Pathology

Comparison of three quantitation methods for PCNA immunostaining: Applicability and relation to survival in 83 astrocytic neoplasms

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Authors

HHHannu HaapasaloTampere University of Applied Sciences PSP SallinenUniversity of Helsinki PHPauli HelénTampere University

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Implication

Cohort study demonstrates that morphometric PCNA quantitation predicts survival in astrocytoma patients, indicating prognostic value independent of tumor grade.

Key Points

To assess three methods of quantifying proliferating cell nuclear antigen (PCNA) immunostaining in astrocytic neoplasms and establish their diagnostic feasibility and correlation with patient survival.
Evaluated 83 routinely fixed and embedded astrocytic neoplasms across malignancy grades I through IV using monoclonal PCNA antibodies and antigen-retrieval techniques.
Quantified proliferation using three techniques: PCNA labeling index (percentage of positive cells), uncorrected index (positive cells per mm2 of tissue), and corrected index (positive cells per mm2 of nuclear area).
Both area-based morphometric indices evaluated approximately 2,000 cells within 7 to 10 minutes, yielding a ten-fold higher throughput than the manual labeling index (200 cells in 7 to 10 minutes).
All three quantification methods significantly correlated with tumor grade (I–IV) and overall patient survival.
The corrected PCNA index split grade IV glioblastoma into two distinct prognostic subgroups, with 1-year survival rates of 56% versus 17%.

Cite This Study

Haapasalo et al. (1993) studied this question.

synapsesocial.com/papers/6a86e25e5ee22a1e35cb9aa1 https://doi.org/10.1002/path.1711710309

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Implication

Cohort study demonstrates that morphometric PCNA quantitation predicts survival in astrocytoma patients, indicating prognostic value independent of tumor grade.

Comparison of three quantitation methods for PCNA immunostaining: Applicability and relation to survival in 83 astrocytic neoplasms

Discussion

Member takes

Implication

Key Points

Cite This Study

Comparison of three quantitation methods for PCNA immunostaining: Applicability and relation to survival in 83 astrocytic neoplasms

Discussion

Member takes

Implication

Key Points

Cite This Study

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