Affinity purification and characterization of salivary components interacting with Streptococcus mutans serotype f 74K SR cell wall protein

Two salivary components which specifically bind to Streptococcus mutans OMZ 175 (serotype f) 74K SR cell surface protein were purified from human whole saliva, free of immunoglobulins, by using affinity chromatography. Both components were eluted from the column in active monomeric forms having Mr of 75 and 60 K. The two binding components were identified by WB analysis as free secretory component (SC) (75K) and as a 60K protein antigenically related to serum albumin (HSA).