Key result
Native platelet-rich plasma yielded significantly higher platelet aggregation compared to platelet count adjusted plasma across all tested ADP concentrations (P<0.0001).
Why the study?
Does the use of native versus adjusted platelet-rich plasma affect light transmittance aggregometry results in stable angina patients on dual antiplatelet therapy?
Observational (n=142)
Does the use of native versus adjusted platelet-rich plasma affect light transmittance aggregometry results in stable angina patients on dual antiplatelet therapy?
The absolute magnitude of platelet aggregation in LTA depends on platelet number, indicating that adjusting PRP platelet counts significantly alters results and may impact the definition of clopidogrel responsiveness.
Adjusting PRP counts may alter LTA-based clopidogrel responsiveness classification in stable angina; leaves open optimal assay standardization for research.
We evaluated whether the results of light transmittance aggregometry (LTA) differ when "native" platelet-rich plasma (PRP) or adjusted (to a standard platelet count of 250.000/microL) PRP is used in patients on dual antiplatelet therapy with aspirin and clopidogrel. LTA has been performed on the blood of 142 stable angina pectoris patients who were adequately pretreated with aspirin and clopidogrel. Platelet aggregation was significant higher in native PRP as compared to platelet count adjusted PRP (P<0.0001) for all four concentrations of adenosine-5'-diphosphate (ADP) (2, 5, 10 and 20 micromol/L). The interindividual variability was significantly higher in platelet count adjusted PRP as compared to native PRP when stimulated with 10 and 20 micromol/L of ADP. The absolute magnitude of aggregation in non-adjusted PRP is clearly dependent on platelet number. These observations are important since several studies have used empirically defined cut-off levels to segregate non-responders from responders to clopidogrel therapy.
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STELT et al. (2007) conducted an observational in Stable angina pectoris (n=142). Native platelet-rich plasma (PRP) vs. Platelet count adjusted PRP (250,000/microL) was evaluated on Platelet aggregation (p=<0.0001). Native platelet-rich plasma yielded significantly higher platelet aggregation compared to platelet count adjusted plasma across all tested ADP concentrations (P<0.0001).
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