Key result
Periostin deficiency significantly reduced cartilage degeneration in mice with post-traumatic osteoarthritis (maximum OARSI score 2.37 vs 4.00, P=0.011) and age-related spontaneous osteoarthritis.
Why the study?
Elevated periostin levels in cartilage and bone are associated with osteoarthritis, but whether periostin loss-of-function can delay or prevent osteoarthritis development was unknown.
Does Postn loss-of-function prevent cartilage degeneration in post-traumatic and age-related osteoarthritis in mice?
Does Postn loss-of-function prevent cartilage degeneration in post-traumatic and age-related osteoarthritis in mice?
Absolute Event Rate: 2.37% vs 4%
p-value: p=0.011
Postn deficiency protects against cartilage degeneration and bone changes in both post-traumatic and age-related osteoarthritis in mice, suggesting Postn as a potential therapeutic target.
Background Elevated levels of periostin (Postn) in the cartilage and bone are associated with osteoarthritis (OA). However, it remains unknown whether Postn loss-of-function can delay or prevent the development of OA. In this study, we sought to better understand the role of Postn in OA development and assessed the functional impact of Postn deficiency on post-traumatic and age-related OA in mice. Methods The effects of Postn deficiency were studied in two murine experimental OA models using Postn −/− ( n = 32) and littermate wild-type ( wt ) mice ( n = 36). Post-traumatic OA was induced by destabilization of the medial meniscus (DMM) in 10-week-old mice ( n = 20); age-related OA was analyzed in 24-month-old mice ( n = 13). Cartilage degeneration was assessed histologically using the OARSI scoring system, and synovitis was evaluated by measuring the synovial lining cell layer and the cells density in the synovial stroma. Bone changes were measured by μCT analysis. Serum levels of Postn were determined by ELISA. Expression of Postn and collagenase-3 (MMP-13) was measured by immunostaining. RNA-seq was performed on chondrocytes isolated from 21-day old Postn −/− ( n = 3) and wt mice ( n = 3) to discover genes and pathways altered by Postn knockout. Results Postn −/− mice exhibited significantly reduced cartilage degeneration and OARSI score relative to wt mice in post-traumatic OA after 8 weeks (maximum: 2.37 ± 0.74 vs. 4.00 ± 1.20, P = 0.011; summed: 9.31 ± 2.52 vs. 21.44 ± 6.01, P = 0.0002) and spontaneous OA (maximum: 1.93 ± 0.45 vs. 3.58 ± 1.16, P = 0.014; summed: 6.14 ± 1.57 vs. 11.50 ± 3.02, P = 0.003). Synovitis was significantly lower in Postn −/− mice than wt only in the DMM model (1.88 ± 1.01 vs. 3.17 ± 0.63; P = 0.039). Postn −/− mice also showed lower trabecular bone parameters such as BV/TV, vBMD, Tb.Th, and Tb.N and high Tb. Sp in both models. Postn −/− mice had negligible levels of serum Postn compared with wt . Immunofluorescent studies of cartilage indicated that Postn −/− mice expressed lower MMP-13 levels than wt mice. RNA-seq revealed that cell-cell-adhesion and cell-differentiation processes were enriched in Postn −/− mice, while those related to cell-cycle and DNA-repair were enriched in wt mice. Conclusions Postn deficiency protects against DMM-induced post-traumatic and age-related spontaneous OA. RNA-seq findings warrant further investigations to better understand the mechanistic role of Postn and its potential as a therapeutic target in OA.
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Attur et al. (2021) studied Osteoarthritis (n=68). Periostin (Postn) loss-of-function (Postn-/-) vs. Wild-type (wt) mice was evaluated on Maximum OARSI score for cartilage degeneration in post-traumatic OA at 8 weeks (p=0.011). Periostin deficiency significantly reduced cartilage degeneration in mice with post-traumatic osteoarthritis (maximum OARSI score 2.37 vs 4.00, P=0.011) and age-related spontaneous osteoarthritis.
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