Answers are grounded in this paper and cite their sources.
A procedure for the isolation of mevalonate and β-hydroxy-β-methylglutarate from animal tissues is presented. This technique involves differential extraction of the short chain acids with ethyl ether, formation of their dibenzylethylenediamine salts, and final purification of the methyl esters of these acids by gas-liquid chromatography. This technique has been employed for the measurement of mevalonate synthesis in a cell-free liver system, and the validation of such a procedure established by showing the chromatographic purity of the methyl and ethyl derivatives of the isolated mevalonate-14C.
No takes yet. Share an insight, caveat, or question.
Siperstein et al. (1966) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: