What question did this study set out to answer?

To examine the subcellular localization and mechanism of Trk receptor transactivation triggered by G-protein-coupled receptor (GPCR) ligands in neuronal cells.

July 28, 2004Journal of NeuroscienceOpen Access

Transactivation of Trk Neurotrophin Receptors by G-Protein-Coupled Receptor Ligands Occurs on Intracellular Membranes

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Authors

RRRithwick RajagopalWashington University in St. Louis ZCZhe-Yu ChenShandong University FLFrancis S. LeeCornell University

Discussion

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Implication

In vitro study shows GPCR ligands transactivate Trk neurotrophin receptors on intracellular Golgi membranes in neuronal cells, indicating neurotrophin-independent signaling occurs internally.

Key Points

To examine the subcellular localization and mechanism of Trk receptor transactivation triggered by G-protein-coupled receptor (GPCR) ligands in neuronal cells.
Treated PC12 cells and primary neurons with GPCR ligands, including adenosine agonists and pituitary adenylate cyclase-activating polypeptide (PACAP), in the presence or absence of transcriptional and translational inhibitors.
Evaluated Trk receptor localization and surface expression using cell-surface biotinylation and immunostaining techniques.
GPCR ligands induced Trk receptor transactivation primarily on intracellular membranes, particularly associating with the Golgi apparatus, rather than at the cell surface.
Unlike standard neurotrophin-mediated activation, GPCR-induced Trk receptor transactivation was sensitive to transcriptional and translational inhibitors.

Cite This Study

Rajagopal et al. (2004) studied this question.

synapsesocial.com/papers/6a86f2acc27ad527967ccbe5 https://doi.org/10.1523/jneurosci.0010-04.2004

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Implication

In vitro study shows GPCR ligands transactivate Trk neurotrophin receptors on intracellular Golgi membranes in neuronal cells, indicating neurotrophin-independent signaling occurs internally.