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Leucyl transfer ribonucleic acid synthetase (EC 6.1.1.4, l-leucine:tRNA ligase (AMP)) from Escherichia coli has been purified to near homogeneity. The molecular weight of the enzyme is approximately 105,000 and its sedimentation coefficient (s020,w) is 5.4 S. In the presence of EDTA or in the absence of reducing agents, the enzyme is rapidly inactivated.
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Hayashi et al. (1970) studied this question.
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