Flagella on Legionnaires' Disease Bacteria in the Human Lung

Brief Reports1 November 1980Flagella on Legionnaires' Disease Bacteria in the Human LungFRANCIS W. CHANDLER, D.V.M., Ph.D., BERENICE M. THOMASON, B.S., G. ANN HÉBERT, B.S.FRANCIS W. CHANDLER, D.V.M., Ph.D.Search for more papers by this author, BERENICE M. THOMASON, B.S.Search for more papers by this author, G. ANN HÉBERT, B.S.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-93-5-715 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptRecently, we observed flagella on several strains of the Legionnaires' disease (LD) bacteriumLegionella pneumophila, grown for 1 to 3 days on charcoal yeast extract (CYE) agar and examined by light and immunofluorescence microscopy (1). Flagella were also easily seen onLegionella micdadei(2, 3) (another name,Legionella pittsburgensis, has been proposed for this organism [4]),Legionella bozemanii(5) andLegionella dumoffii(5, 6) grown on CYE agar. Unlike Mueller-Hinton agar (7), CYE agar stimulates early profuse growth of the bacterium, a condition necessary for optimal flagellar synthesis (8). Because flagella were seen onL. pneumophilain vitro, we have...References1. THOMASON B, CHANDLER F, and HOLLIS D. Flagella on Legionnaires' disease bacteria: an interim report. Ann Intern Med. 1979;91:224-6. LinkGoogle Scholar2. HÉBERT G, MOSS C, MCDOUGAL L, BOZEMAN F, MCKINNEY R, and BRENNER D. The rickettsia-like organism TATLOCK (1943) and HEBA (1959): bacteria phenotypically similar to but genetically distinct from Legionella pneumophila and the WIGA bacterium. Ann Intern Med. 1980;92:45-52. LinkGoogle Scholar3. HEBERT G, STEIGERWALT A, and BRENNER D. Legionella micdadei species nova: classification of a third species of Legionella associated with human pneumona. Curr Microbiol. 1980;3:255-8. CrossrefGoogle Scholar4. PASCULLE A, FEELEY J, and GIBSON R. Pittsburgh pneumonia agent: direct isolation from human lung tissue. J Infect Dis. 1980;141:727-32. CrossrefMedlineGoogle Scholar5. BRENNER D, STEIGERWALT A, and GORMAN G. Legionella bozemanii species nova and Legionella dumoffii species nova: classification of two additional species of Legionella associated with human pneumonia. Curr Microbiol. 1980;4:111-6. CrossrefGoogle Scholar6. LEWALLEN K, MCKINNEY R, and BRENNER D. A newly identified bacterium phenotypically resembling, but genetically distinct from, Legionella pneumophila: an isolate in a case of pneumonia. Ann Intern Med. 1979;91:831-4. LinkGoogle Scholar7. FEELEY J, GORMAN G, and GIBSON R. Primary isolation media and methods. In: JONES GL, HEBERT GA, eds. "Legionnaires": The Disease, the Bacterium and Methodology. Atlanta: Center for Disease Control; 1979:77-84. Google Scholar8. LEIFSON E. Atlas of Bacterial Flagellation. New York: Academic Press; 1960:1. CrossrefGoogle Scholar9. CLARK W. A simplified Leifson flagella stain. J Clin Microbiol. 1976;3:632-4. CrossrefMedlineGoogle Scholar10. CHANDLER F, HICKLIN M, and BLACKMON J. Demonstration of the agent of Legionnaires' disease in tissue. N Engl J Med. 1977;297:1218-20. CrossrefMedlineGoogle Scholar11. CHANDLER F, BLACKMON J, HICKLIN M, COLE R, and CALLAWAY C. Ultrastructure of the agent of Legionnaires' disease in the human lung. Am J Clin Pathol. 1979;71:43-50. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: FRANCIS W. CHANDLER, D.V.M., Ph.D.; BERENICE M. THOMASON, B.S.; G. ANN HÉBERT, B.S.Affiliations: Center for Disease Control Atlanta, Georgia▸Requests for reprints should be addressed to Francis W. Chandler, D.V.M.; Histopathology Laboratory, Pathology Division, Bureau of Laboratories, Center for Disease Control; Atlanta, GA 30333. 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