A comparative study of the acute and long-term prognosis for mouse models undergoing laparoscopic surgery under continuous intra-abdominal perfusion with either CO₂ gas or saline

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Background: We developed a water-filled laparoendoscopic surgery (WaFLES) method using an isotonic irrigant like physiological saline instead of carbon dioxide (CO2) insufflation. Although surgical experimentation in porcine models has revealed some advantages, the effect of perfusate absorption remains uncertain. Here, we examined the acute and long-term prognosis of this method in mouse models. Materials and methods: CO2 gas or physiological saline was continuously perfused into mice for 1 h. Body-weight fluctuation was observed for 100 days in the two groups and control anesthesia group of mice. In addition, the induction of stress proteins and cytokines was evaluated immediately after perfusion. Results: Mice perfused with saline showed a temporary 30% increase in body weight during perfusion; however, this increase was not significant when assessed one day later. There was no significant increase in either IL6 or TNFα levels in the peritoneal lavage fluid obtained from any of the three groups. There were no significant changes in the expression of HSP70 or BiP. All mice survived over the long-term observation period of 100 days without any evidence of body-weight fluctuation (P = 0.7408, N = 5 for each group). Conclusion: WaFLES showed a good prognosis in a mouse model, thus indicating significant potential for clinical application.

Continuous intra-abdominal perfusion with physiological saline is safe and well-tolerated in a mouse model, suggesting potential for clinical application in laparoendoscopic surgery.