Perfusing isolated rat hearts with hydrogen peroxide: an experimental model of cardiac dysfunction caused by reactive oxygen species
Population
Isolated, Langendorff-perfused rat hearts (n=62)
Comparison
Perfusion with hydrogen peroxide 180 µmol l-1… vs Control perfusion alone or with antioxidants
Design
Preclinical
Follow-up
60 minutes (10 min perfusion followed by 50 min recovery)
Authors
Discussion
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Member takes
Implication
H2O2 models reversible LV dysfunction in isolated rat hearts; leaves open whether ROS scavenging improves clinical outcomes.
Structured PICO
P
Population
Isolated, Langendorff-perfused rat hearts (n=62)
I
Intervention
Perfusion with hydrogen peroxide (H2O2) 180 µmol l-1 for 10 min followed by recovery for 50 min, alone or with antioxidants (N-acetylcysteine, thiourea, catalase, deferoxamine)
C
Comparator
Control perfusion alone or with antioxidants
O
Outcome
Coronary flow (CF), left ventricular developed pressure (LVDP), left ventricular end-diastolic pressure (LVEDP), and heart rate (HR)surrogate
H2O2 perfusion provides a convenient and reversible experimental model of ROS-induced functional injury in isolated rat hearts, primarily mediated by H2O2 rather than the hydroxyl radical.
Cite This Study
Skjelbakken et al. (1996) studied this question.