What question did this study set out to answer?

To determine whether macrophage colony-stimulating factor and related hematopoietic cytokines enhance antibody-dependent cellular cytotoxicity in monocytes and macrophages.

March 1, 1989Cellular ImmunologyOpen Access

Macrophage colony-stimulating factor enhances monocyte and macrophage antibody-dependent cell-mediated cytotoxicity

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Authors

RMR. Allan MufsonNational Cancer Institute JAJ. D. AghajanianWyeth GWGordon WongUniversity Health Network

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Implication

In vitro study demonstrates that M-CSF enhances antibody-dependent cytotoxicity in monocytes and macrophages, suggesting potential to improve monoclonal antibody cancer therapies.

Key Points

To determine whether macrophage colony-stimulating factor and related hematopoietic cytokines enhance antibody-dependent cellular cytotoxicity in monocytes and macrophages.
Cultured human peripheral blood monocytes and murine peritoneal macrophages in vitro for 72 hours with M-CSF, GM-CSF, or IL-3.
Assayed antibody-dependent cellular cytotoxicity (ADCC) across multiple effector-to-target ratios and concentrations of tumor-specific monoclonal antibodies.
Quantified monocyte TNF-alpha secretion and evaluated the effect of neutralizing anti-TNF-alpha antibodies on ADCC activity.
M-CSF treatment substantially enhanced ADCC in human monocytes and murine macrophages, enabling effective cytotoxicity at lower effector-to-target ratios and lower antibody concentrations.
Neither GM-CSF nor IL-3 augmented ADCC in cultured monocytes, although all three tested cytokines increased monocyte TNF-alpha release.
ADCC enhancement required lower M-CSF concentrations (1–10 ng/ml) than TNF-alpha induction (20–100 ng/ml), and neutralizing TNF-alpha antibodies did not diminish ADCC.

Cite This Study

Mufson et al. (1989) studied this question.

synapsesocial.com/papers/6a8714c048ef9afcd2a25d88 https://doi.org/10.1016/0008-8749(89)90234-7

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Implication

In vitro study demonstrates that M-CSF enhances antibody-dependent cytotoxicity in monocytes and macrophages, suggesting potential to improve monoclonal antibody cancer therapies.

Macrophage colony-stimulating factor enhances monocyte and macrophage antibody-dependent cell-mediated cytotoxicity

Discussion

Member takes

Implication

Key Points

Cite This Study

Macrophage colony-stimulating factor enhances monocyte and macrophage antibody-dependent cell-mediated cytotoxicity

Discussion

Member takes

Implication

Key Points

Cite This Study

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