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Bovine serum albumin and chicken-egg lsoyzyme can be cleaved with high specificity into two fragments by the method shown below. A pyrene-L-phenylalanine conjugate was used as the probe (shown schematically over the first arrow), which binds with site specificity to the protein and produces the cleavage upon irradiation with visible light in the presence of a CoIII compound.
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Kumar et al. (1997) studied this question.
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