The Inhibition of Rat Liver Polyribosome Breakdown in the Presence of Liver Supernatant

Rat liver ribosomes prepared from the rapidly sedimenting pellet of a liver homogenate or from the microsomal pellet by the treatment of these pellets with sodium deoxycholate in buffer are deficient in polyribosomes and contain primarily ribosome dimers. Dimer formation can be prevented and a good polyribosome pattern can be obtained from both these pellets provided treatment with sodium deoxycholate takes place in the presence of liver supernatant. It has been shown that the polyribosomes isolated from the rapidly sedimenting pellet incorporate glycine into acid-insoluble material in vivo and are broken down into single ribosomes and ribosome dimers by ribonuclease.