July 21, 2011Journal of Biological ChemistryOpen Access

Structural and Biochemical Analysis of Nuclease Domain of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Protein 3 (Cas3)

View Full Paper

Ask AI

Bookmark

Population

Thermus thermophilus HB8 Cas3 HD domain

Design

Preclinical

Authors

SMSabin MulepatiJohns Hopkins University SBScott BaileyJohns Hopkins University

Discussion

Member takes

Implication

Elucidates Cas3 metal specificity in CRISPR; leaves open translation to optimized gene-editing tools.

Structured PICO

Population

Thermus thermophilus HB8 Cas3 HD domain

Intervention

Structural and biochemical analysis, structure-guided mutagenesis

Outcome

Crystal structure of the HD domain of Cas3, metal ion binding, and endonuclease activity activationsurrogate

The study provides the crystal structure and biochemical characterization of the Cas3 HD domain, elucidating its metal-dependent endonuclease activity essential for CRISPR-mediated protection.

Cite This Study

Mulepati et al. (2011) studied this question.

synapsesocial.com/papers/6a8727aa540b033b21bf0fb1 https://doi.org/10.1074/jbc.m111.270017

View Full Paper

Ask AI

Bookmark

Structural and Biochemical Analysis of Nuclease Domain of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Protein 3 (Cas3)

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Structural and Biochemical Analysis of Nuclease Domain of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Protein 3 (Cas3)

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider