Demonstration of Rna Synthesis Caused by Japanese Encephalitis Virus Infection in Ps (Y-15) Cells With the AId of Chromomycin A3

PS (Y-15) cells, a porcine kidney stable cell line, were infected with Japanese encephalitis virus (JEV), a member of RNA-containing viruses. Immediately after virus adsorption, the cells were treated with chromomycin A3 in a concentration sufficient to suppress cellular RNA synthesis. The synthesis of viral particles and the production of viral antigen in chromomycin-treated cells were similar to those in non-treated controls in respect to the rate as well as the time course. The results suggest that chromomycin A3 dose not inhibit RNA synthesis by JEV infection. Eventually, RNA synthesis by JEV infection under the condition in which cellular RNA synthesis was completely suppressed by chromomycin A3 was demonstrated autoradiographically to occur in the nucleus several hours prior to the production of viral particles.