The objectives of this work were to: (1) contrast spray drying, freeze drying and freezing for large‐scale preservation of B. linens , (2) determine the thermal resistance curves, and (3) measure the storage stability. When B. linens was freeze‐dried and frozen in feed suspensions containing 3% (w/v) cell paste and 25% (w/w) total solids, survival was 100%. During spray drying, lethal thermal injury was the main cause of loss of viability. Accordingly, by extrapolation, 100% viability would be possible at an outlet‐air temperature of 57°C. Spray‐dried and freeze‐dried cells were stable during storage at 4°C in the absence of oxygen and moisture.
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To et al. (1997) studied this question.
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