RBM45: Molecular, Cellular, and Evolutionary Biology

polyadenylation consensus signal.Downstream of the polyadenylation consensus signal is a polyadenylation cleavage site and a U-rich motif.All the exon-intron junctions conformed to consensus splice donor/acceptor sites.Control modules for transcription could be elucidated by fusing the RBM45 5ʹ-UTR to a reporter gene such as luciferase or green fluorescent protein and assaying function of deletion and point mutations in yeast and/or cell culture.Concurrently, candidate transcription factor binding sites in the 5ʹ-UTR could be identified by bioinformatics and protein binding evaluated by electrophoretic mobility shift assay.