A Planar Quartz Waveguide Immunosensor Based On TIRF Principle

An immunosensor based on TIRF principle for monitoring antibody-antigen reactions at solution-quartz interface was developed. The human IgG (as antigen) was immobilized on the surface of a planar quartz waveguide. Binding of FITC labelled goat anti-human IgG from solution to the immobilized antigen was detected, and non-bound signal, non-specific and specific binding singals were distinguished. Four dissociation solutions were used to invwtigate the regeneration of the solid phase p r e tein by monitoring the Ag-Ab dissociation. The immuno-competitive assay was described, which showed the dynamic detecting range could be adjusted. The sensitivity of the sensor system was also given.